Human Adipokine Antibody Array

• Human Adipokine Antibody Array, Ordering Information

Introduction

R&D Systems Human Adipokine Array (Catalog # ARY024) is a rapid, sensitive, and economical tool used to simultaneously detect the relative levels of 58 different obesity-related molecules in a single sample. No specialized equipment is necessary

• Product Insert
• Troubleshooting Guide
• Assays for analytes represented in the Human Adipokine Array Kit

General Assay Principle

Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernatant, cell lysate, tissue lysate, serum, plasma, saliva, urine, or human milk samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any cytokine/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of cytokine bound. Chemiluminescence is detected in the same manner as a Western blot.

Kit Contents

• 4 Array Membranes
• 4-Well Multi-dish
• Array Buffers
• Wash Buffer
• Detection Antibody Cocktail
• Streptavidin-HRP
• Chemiluminescent Detection Reagents
• Transparency Overlay Template
• Detailed Protocol

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Materials Required sections of the product datasheet.